Leerdoelen voor de cursus
At the end of the course, students should have a profound understanding of:
· NMR and its applications in life sciences;
· the range of samples that can be studies by either solution or solid-state NMR;
· the relation between NMR parameters and molecular structure and dynamics.
After completing the course the student is able to:
· describe and identify the different elements of an NMR experiment (solution and solid-state);
· describe the underlying concept of the product operator formalism;
· use the product operator formalism to analyze solution-state NMR experiments;
· describe how high-resolution spectra of solids can be obtained;
· describe the diverse methods of sensitivity improvement;
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Description of content
The nuclear spin is the ultimate spy. Nuclear magnetic resonance exploits the spin’s special properties to study molecular structure and dynamics in a non-invasive way. Key herein is control over the state of the spin. But how do you exercise such control? How do you exploit such control to extract information on the molecule’s structure and motions?
This course will cover the principles of both solid and solution-state NMR and its application to biomolecules. Emphasis will be on a conceptual and practical understanding of NMR theory and its application to current challenges in structural biology.
The course will consist of lectures, exercises, a paper presentation, a computer practical and a practical at the NMR machine (depending on the number of students). The paper presentation will count 20% and the exam 80% towards the final grade.
The course is scheduled to last two-weeks with no lectures on Mondays. The first week covers solution-NMR, the second week solid-state NMR. The students will also study an paper which they present towards the end of the course.
Course Programme
Day 1 |
Morning |
General Introduction
Introduction to Product Operator Formalism (POF) |
Afternoon |
Preparation for computer practicals and exercises |
Day 2 |
Morning |
Analysis of HSQC/HMQC using POF |
Afternoon |
Exercises and computer practical |
Day 3 |
Morning |
Analysis of triple-resonance experiments using POF |
Afternoon |
Exercises and computer practical |
Day 4 |
Morning |
Conformational dynamics by NMR |
Afternoon |
Exercises and computer practical |
Day 5 |
Morning |
Introduction to solid-state NMR (ssNMR) |
Afternoon |
Exercises and computer practical |
Day 6 |
Morning |
Biological solid-state NMR |
Afternoon |
Exercises and computer practical |
Day 7 |
Morning |
Applications of ssNMR in challenging biological systems |
Afternoon |
NMR practical |
Day 8 |
Morning |
Self Study, Q&A (ssNMR) |
Afternoon |
DNP-enhanced solid-state NMR |
Day 9 |
Morning |
Preparation presentations |
Afternoon |
Paper presentations |
Day 10 |
Morning |
Self study, Q&A |
Afternoon |
Self study |
Day 11 |
Morning |
Self study |
Afternoon |
Final exam |
Literature/study material used:
Recommended book for further in depth reading:
· J. Keeler, Understanding NMR Spectroscopy, 2nd edition, Wiley, 2006.
Handouts of the lectures, material for the computer practicals and exercise session will be provided in class.
The students should bring their own laptop for the practicals.
Registration:
Osiris Student
Mandatory for students in Master’s programme: NO.
Optional for students in other Master’s programmes GS-LS: YES.
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