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Cursus: B-B3LEM18
B-B3LEM18
Licht en electronen microscopie
Cursus informatieRooster
CursuscodeB-B3LEM18
Studiepunten (ECTS)7,5
Categorie / Niveau3 (Bachelor Gevorderd)
CursustypeCursorisch onderwijs
VoertaalEngels
Aangeboden doorFaculteit Betawetenschappen; Undergraduate School Bètawetenschappen;
Contactpersoondr. L.C. Kapitein
E-maill.kapitein@uu.nl
Docenten
Docent
dr. L.C. Kapitein
Overige cursussen docent
Contactpersoon van de cursus
dr. L.C. Kapitein
Overige cursussen docent
Docent
dr. Z Zeev Ben Mordehai
Overige cursussen docent
Blok
2  (12-11-2018 t/m 01-02-2019)
Aanvangsblok
2
TimeslotBC: Zie 'Help'
Onderwijsvorm
Voltijd
Cursusinschrijving geopendvanaf 17-09-2018 t/m 30-09-2018
AanmeldingsprocedureOsiris
Inschrijven via OSIRISJa
Inschrijven voor bijvakkersNee
VoorinschrijvingNee
WachtlijstNee
Cursusdoelen
Leerdoelen - course objectives
At the end of this course you will:
  • know the physical principles underlying light and electron microscopy
  • have a deep understanding of the workings of modern light and electron microscopes
  • understand the principles of specialized light microscopy techniques, such as confocal microscopy, TIRF microscopy, Selective Plane Illumination Microscopy, Super-resolution microscopy
  • know how to choose between different labeling strategies to generate fluorescence contrast
  • know how to prepare samples for an electron microscope
  • be familiar with the different applications of light and electron microscopy
  • be able to critically read scientific papers where LM and/or EM were used.
Vaardigheden - Skills
  • Hands-on experience with setting up optical systems
  • Hands-on experience with performing image analysis
  • Hands-on experience with sample preparation for EM and using EM.
  • Analysing primary literature and presenting it.
  • Critical thinking
Inhoud
Studiepad
This course fits within the study path Cell Biology. It provides a good preparation for the track Molecules and Cells and the track Biophysics and Molecular Imaging in the Master program Molecular and Cellular Life Sciences.

Inhoud - Content
The aim of this course is to provide the students with deep understanding of modern light and electron microscopy. The emphasis will be on the applications for cell biology and structural biology.
The course is divided in two part. The first part cover light microscopy and the second part electron microscopy.
The course will consists of lectures (HC) covering the theory accompanied by tutorials (WC). In additions, the course will provide practicals where the students will have the opportunity to get hands-on experience with or demonstrations of the different microscopes.
 
Werkvormen
Hoorcolleges
Werkcolleges
Practica
Journal club
Zelfstudie
Schrijfopdracht
 
Toetsing - assessment
The grade for the course will be the average of the separate grades for LM and EM. Each of these two grades should be a 5 or higher to pass.
For the LM part:
·         Written exam (80%)
In addition, attendance and active participation of the Werkcolleges and Practicals is obligatory and will be registered (inspanningsverplichting). You cannot pass the course if you were absence without prior approval.
Studiemateriaal
LM part
  • Murphy and Davidson, Fundamental of Light Microscopy and Electronic Imaging.
Online available through the UU library

recommended additional reading
  • Kubitscheck (editor), Fluorescence Microscopy - From Principles to Biological Application. Digitaal beschikbaar via de UU bibliotheek:
Online available through the UU library
  • Manual practicals
  • Manual image analysis
EM part
  • Selected chapters from the book “Transmission Electron Microscopy” by Williams and Carter (ISBN 9780387765020)
  • Lecture slides
  • Manual practicals
Ingangseisen
Je moet ingeschreven staan voor één van de volgende opleidingen:
- Biologie
- Scheikunde
Voorkennis
The level 2 course Moleculaire genetische onderzoekstechnieken (B-B2MGOT14) or comparable knowledge (MLS=Mol. biol. en biochem. technieken (SK-B2MBBT)) is a prerequisite. The courses Math and Physics 2 (SK-BWSNK2) and de Cel (B-B2CEL09) or comparable (MLS=Moleculaire Celbiologie (B-B1MOL14)) are recommended.
Verplicht materiaal
Boek
Murphy and Davidson, Fundamental of Light Microscopy and Electronic Imaging. Online available through the UU library http://onlinelibrary.wiley.com/book/10.1002/9781118382905
Boek
Kubitscheck (editor), Fluorescence Microscopy - From Principles to Biological Application. Digitaal beschikbaar via de UU bibliotheek: Online available through the UU library http://onlinelibrary.wiley.com/book/10.1002/9783527671595
Aanbevolen materiaal
Boek
Transmission Electron Microscopy” by Williams and Carter (ISBN 9780387765020)
Werkvormen
Hoorcollege

Practicum

Werkcollege

Toetsen
Eindresultaat
Weging100
Minimum cijfer-

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